Basic Information
Regular Relationship :
Tissue/Cell lineOsteosarcoma Cells
SpecieHomo sapiens (human)
CitationAm J Transl Res. 2021 Jul 15;13(7):7783-7793. eCollection 2021.
Reference title
LncRNA HCG18 promotes cell multiplication and metastasis by miR-148b/ETV5 regulation in osteosarcoma.
Experimental verification
MTT assay;qPCR;RT-qPCR;Western blot;Luciferase reporter assay;MTT assay;
Functional description
OBJECTIVE: To determine the effects of long non-coding RNA (LncRNA) HCG18 on cell multiplication and invasion of osteosarcoma. METHODS: MTT assay and transwell assay were used for cell multiplication and invasion, respectively. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot were used to determine transcriptional and translational expression. Luciferase reporter assay was used to identify the specific target relationships. RESULTS: The expression of LncRNA HCG18 was dramatically increased in osteosarcoma cells compared to the normal tissues. LncRNA-HCG18 accelerated cell multiplication and invasion in vitro, which was achieved by down-regulating the overexpression of miR-148b, and down-regulating ETV5, indicating combination of ETV5 and miR-148b in osteosarcoma. Overexpression of ETV5 could reverse the inhibitory effect of knockout of lncRNA HCG18 on cell multiplication and invasion. CONCLUSION: LncRNA HCG18 acted as a sponge of miR-148b and played an oncogenic role in osteosarcoma, providing therapeutic targets for osteosarcoma.
Annotations
External Annotation for HCG18 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
