Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieColorectal Cancer
CeRNA1CDKN2B-AS1[LncRNA]
miRNAmiR-28-5p[miRNA]
CeRNA2URGCP[mRNA]
Tissue/Cell lineColorectal Cancer Tissues
SpecieHomo sapiens (human)
CitationOncol Rep. 2021 Oct;46(4):213. doi: 10.3892/or.2021.8164. Epub 2021 Aug 9.
Reference title
LncRNA CDKN2B-AS1 sponges miR-28-5p to regulate proliferation and inhibit apoptosis in colorectal cancer.
Experimental verification
Luciferase reporter assay;
Functional description
Long noncoding RNA (lncRNA) CDKN2B-antisense RNA 1 (AS1) functions as a tumor oncogene in numerous cancers. However, the roles and mechanism of CDKN2B-AS1 in colorectal cancer (CRC) have not been explored. The present study aimed to investigate whether and how CDKN2B-AS1 contributes to CRC progression. The data revealed that CDKN2B-AS1 expression was upregulated in CRC tissues. Loss-of-function assays demonstrated that CDKN2B-AS1 in CRC modulated cell proliferation and apoptosis, which was mediated by cyclin D1, cyclin-dependent kinase (CDK) 4, p-Rb, caspase-9 and caspase-3. Bioinformatics analysis and luciferase reporter assays indicated direct binding of microRNA (miR)-28-5p to CDKN2B-AS1. Moreover, the results herein revealed that the expression of miR-28-5p was negatively correlated with that of CDKN2B-AS1 in CRC tissue. Moreover, CDKN2B-AS1 acted as a miR-28-5p competing endogenous RNA (ceRNA) to target and regulate the expression of URGCP. These findings indicated that CDKN2B-AS1 plays roles in CRC progression, providing a potential therapeutic target or novel diagnostic biomarker for CRC.
Annotations
External Annotation for CDKN2B-AS1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
