Basic Information
Regular Relationship :
Tissue/Cell lineL428 Hodgkin Lymphoma Cell Line,Hek293 And U87Mg Glioblastoma Cells
SpecieMus musculus (mouse)
CitationSci Rep 2013 3, 2535 doi:10.1038/srep02535 PMID:23985560
Reference title
microRNA-9 targets the long non-coding RNA MALAT1 for degradation in the nucleus
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays;RNA immunoprecipitation
Functional description
Growing evidence supports an important role for miR-9 in tumorigenesis16,17. In order to gain insights into miR-9 mechanisms of action we performed RNA-seq transcriptome analyses in the L428 Hodgkin Lymphoma cell line transfected with an LNA-modified inhibitor of miR-9 or a scrambled control. Among the upregulated transcripts, MALAT1 was found to be >16 fold upregulated. To further confirm that the miR-9:MALAT1 interaction occurs through the predicted miR-9 binding sites, we cloned two fragments of MALAT1 encompassing the two putative binding sites downstream the luciferase gene and performed luciferase assays in U87MG cells transfected with reporter plasmids containing the two putative miR-9 binding sites. In both cases, miR-9 over-expression resulted in a significant decrease in luciferase activity, while an opposite effect was observed upon miR-9 inhibition. Moreover, directed mutagenesis of the predicted miR-9 binding sites abolished this effect.
Annotations
External Annotation for MALAT1 |
|
|---|---|
 |
LncRNA-associated competing triplets and functions. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
 |
Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
 |
Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
 |
Information on all annotated and predicted human genes. |
 |
Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
 |
Genome browser for vertebrate genomes. |
 |
An annotated collection of all publicly available DNA sequences. |
 |
A wiki-based platform for community curation of human long non-coding RNAs. |
 |
An integrated knowledge database dedicated to non-coding RNAs. |
 |
An integrated database of human annotated lncRNA transcripts. |
 |
Comprehensive annotations of eukaryotic long non-coding RNAs. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
 |
The catalogue of somatic mutations in cancer. |
