Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieMultiple Myeloma
CeRNA1DANCR[LncRNA]
miRNAmiR-135b-5p[miRNA]
CeRNA2KLF9[mRNA]
Tissue/Cell lineMultiple Myeloma Cells
SpecieHomo sapiens (human)
CitationMol Med Rep. 2021 Sep;24(3):649. doi: 10.3892/mmr.2021.12288. Epub 2021 Jul 19.
Reference title
Long non-coding RNA DANCR represses the viability, migration and invasion of multiple myeloma cells by sponging miR-135b-5p to target KLF9.
Experimental verification
Dual-luciferase reporter assay;MTT assay;Western blot;Luciferase reporter assay;MTT assay;
Functional description
Multiple myeloma (MM) is a malignancy of plasma cells that leads to marrow failure and bone lesions. Numerous studies have verified the link between long non-coding RNAs (lncRNAs) and MM. The present study aimed to examine the role and underlying mechanism of differentiation antagonizing non-protein coding RNA (DANCR) in MM cells. The relative expression levels of DANCR, microRNA (miR)-135b-5p and Krüppel-like factor 9 (KLF9) were examined using reverse transcription-quantitative PCR. Cell viability was assessed using the MTT assay, while relative cell migration and invasion were evaluated using Transwell assays. Moreover, the dual-luciferase reporter assay was used to examine the interplay between DANCR, miR-135b-5p and KLF9. Western blotting was performed to determine the expression level of KLF9. It was found that lncRNA DANCR and KLF9 were downregulated, while miR-135b-5p was upregulated in the serum of patients with MM and in MM cells compared with the controls. Overexpressing DANCR or knocking down miR-135b-5p reduced the viability of the MM cells, as well as restrained MM cells from migrating and invading. Furthermore, DANCR directly targeted miR-135b-5p and was negatively correlated with miR-135b-5p. It was also found that KLF9 was targeted by miR-135b-5p and was inversely correlated with miR-135b-5p expression. The impact of lncRNA DANCR-mediated suppression on cell viability, invasion and migration was partially abolished by short hairpin RNA KLF9 or miR-135b-5p mimics transfection in MM cells. Thus, it was suggested that lncRNA DANCR repressed the viability, migration and invasion of MM cells by sponging miR-135b-5p to target KLF9.
Annotations
External Annotation for DANCR |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
