Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieNasopharyngeal Cancer
CeRNA1FBXL19-AS1[LncRNA]
miRNAmiR-431[miRNA]
CeRNA2PBOV1[mRNA]
Tissue/Cell lineNpc Tissues And Cells
SpecieHomo sapiens (human)
CitationMol Med Rep. 2021 Sep;24(3):647. doi: 10.3892/mmr.2021.12286. Epub 2021 Jul 19.
Reference title
FBXL19-AS1 promotes the progression of nasopharyngeal carcinoma by acting as a competing endogenous RNA to sponge miR-431 and upregulate PBOV1.
Experimental verification
qRT-PCR
Functional description
Long non-coding RNAs (lncRNAs) have been shown to function as crucial regulators in the progression of various types of cancer, including nasopharyngeal carcinoma (NPC). The aim of the present study was to investigate the mechanisms underlying the role of the FBXL19-AS1/microRNA (miR)-431/prostate and breast cancer overexpressed 1 (PBOV1) axis in the progression of NPC. The expression levels of FBXL19-AS1, miR-431 and PBOV1 were assessed by reverse transcription-quantitative PCR. The Cell Counting Kit-8 assay was utilized to detect cell viability. Cell migration and invasion were determined using a Transwell assay. The associations between FBXL19-AS1 and miR-431 or miR-431 and PBOV1 were verified via bioinformatics analysis, dual-luciferase and RNA-binding protein immunoprecipitation assays. It was demonstrated that the expression levels of FBXL19-AS1 and PBOV1 were upregulated in NPC tissues and cells, whereas miR-431 expression was downregulated. FBXL19-AS1 directly interacted with miR-431. FBXL19-AS1 silencing inhibited the viability, migration and invasion of C666-1 and SUNE1 cells, whereas these effects could be alleviated by suppressing miR-431. miR-431 could target the 3'-untranslated region of PBOV1. Overexpression of PBOV1 neutralized the miR-431-mediated suppression of NPC progression. Moreover, FBXL19-AS1 could regulate PBOV1 by sponging miR-431 in NPC cells. In conclusion, the lncRNA FBXL19-AS1 accelerated NPC progression via the miR-431/PBOV1 axis, suggesting that it may serve as a potential therapeutic target for patients with NPC.
Annotations
External Annotation for FBXL19-AS1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
