Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieCoronary Artery Disease
CeRNA1uc003pxg.1[LncRNA]
miRNAmiR-25-5p[miRNA]
CeRNA2cyclin D1[mRNA]
Tissue/Cell lineHuman Umbilical Vein Endothelial Cell
SpecieHomo sapiens (human)
CitationInt J Mol Med. 2021 Aug;48(2):160. doi: 10.3892/ijmm.2021.4993. Epub 2021 Jul 2.
Reference title
Long noncoding RNA uc003pxg.1 regulates endothelial cell proliferation and migration via miR-25-5p in coronary artery disease.
Experimental verification
Dual-luciferase reporter assay;qPCR;RT-qPCR;Western blot;Luciferase reporter assay;
Functional description
Long noncoding RNAs (lncRNAs) have been reported to be associated with the progression of coronary artery disease (CAD). In our previous study, the levels of lncRNA uc003pxg.1 were upregulated in patients with CAD compared with those in control subjects. However, the role and underlying mechanism of the effects of uc003pxg.1 in CAD remain unknown. Therefore, the aim of the present study was to investigate the expression pattern and biological function of uc003pxg.1 in CAD. First, uc003pxg.1 expression levels were assessed in peripheral blood mononuclear cells isolated from patients with CAD by reverse transcription-quantitative (RT-q)PCR. The results demonstrated that the levels of uc003pxg.1 were significantly upregulated (~4.6-fold) in samples from 80 patients with CAD compared with those in 80 healthy subjects. Subsequently, the present study demonstrated that small interfering RNA-mediated uc003pxg.1 knockdown inhibited human umbilical vein endothelial cell (HUVEC) proliferation and migration, which was analyzed using the Cell Counting Kit-8, cell cycle, EdU and Transwell assays. Additionally, the results of RT-qPCR and western blot analyses revealed that uc003pxg.1 regulated the mRNA and protein levels of cyclin D1 and cyclin-dependent kinase. Through high-throughput sequencing and dual-luciferase reporter assays, the present study demonstrated that microRNA (miR)-25-5p was a downstream target of uc003pxg.1. Further experiments verified that uc003pxg.1 regulated HUVEC proliferation and migration via miR-25-5p. The results of the present study may enhance the current understanding of the role of lncRNA uc003pxg.1 in CAD.
Annotations
External Annotation for uc003pxg.1 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
