Basic Information
Regular Relationship :
Tissue/Cell lineDu145, Hct116
SpecieHomo sapiens (human)
CitationCell 2011 Oct 14 147, 344-57 doi:10.1016/j.cell.2011.09.029 PMID:22000013
Reference title
Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
We investigated the ability of these putative PTEN ceRNAs to modulate PTEN levels by first examining the effect of depletion of these candidates on endogenous PTEN protein levels in DU145 prostate cancer cells. For this analysis, we also included two genes, ACSL4 and UNC5CL, which are not predicted targets of these PTEN-targeting microRNAs as negative controls. These experiments were per- formed using siRNA pools (a combination of four independent siRNAs), which are designed to achieve strong on-target gene knockdown with minimal off-target effects. Real-time PCR analysis confirmed efficient siRNA-mediated knockdown of candidate PTEN ceRNAs. Depletion of SERINC1, ZNF460, VAPA,or CNOT6L transcripts did indeed result in a significant reduction in PTEN protein levels, whereas depletion of ACSL4 or UNC5CL did not have a significant effect.
Annotations
External Annotation for ZNF460 |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
