Basic Information
Regular Relationship :
Tissue/Cell lineA375, Wm35, 451Lu And Wm278
SpecieHomo sapiens (human)
CitationCell 2011 Oct 14 147, 382-95 doi:10.1016/j.cell.2011.09.032 PMID:22000016
Reference title
In vivo identification of tumor-suppressive PTEN ceRNAs in an oncogenic BRAF-induced mouse model of melanoma
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
To examine whether the identified putative PTEN ceRNAs regulate expression of PTEN, we performed RNAi-mediated gene silencing in human melanoma cells using pools of four siRNAs to reduce off-target effects. We selected eight putative PTEN ceRNAs (AFF1, DCBLD2, JARID2, MBNL1, RBM9, TNRC6a, TNRC6b, and ZEB2) and depleted them in WM35 cells. Knockdown of seven genes led to reduction of PTEN, six of which significantly attenuated PTEN expression (AFF1, JARID2, MBNL1, RBM9, TNRC6a, TNRC6b, and ZEB2). A reduction of PTEN mRNA levels following PTEN ceRNA silencing was also evident in WM35 cells.
Annotations
External Annotation for TNRC6a |
|
|---|---|
 |
LncRNA-associated competing triplets and functions. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
 |
Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
 |
Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
 |
Information on all annotated and predicted human genes. |
 |
Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
 |
Genome browser for vertebrate genomes. |
 |
An annotated collection of all publicly available DNA sequences. |
 |
A wiki-based platform for community curation of human long non-coding RNAs. |
 |
An integrated knowledge database dedicated to non-coding RNAs. |
 |
An integrated database of human annotated lncRNA transcripts. |
 |
Comprehensive annotations of eukaryotic long non-coding RNAs. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
 |
The catalogue of somatic mutations in cancer. |
