Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieAlzheimers Disease
CeRNA1miR-188-3p sponge[Artifically engineered RNA]
miRNAmiR-188-3p[miRNA]
CeRNA2BACE1[mRNA]
Tissue/Cell lineBrain Tissues
SpecieMus musculus (mouse)
CitationJ Neurosci 2014 Nov 5 34, 14919-33 doi:10.1523/jneurosci.1165-14.2014 PMID:25378159
Reference title
Synaptic and Cognitive Improvements by Inhibition of 2-AG Metabolism Are through Upregulation of MicroRNA-188-3p in a Mouse Model of Alzheimer's Disease.
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
To further determine whether the effect of 2-AG on BACE1 expression is mediated through miR-188-3p,weusedthemiRNA“sponge�technique,which renders a continuous miRNA loss of function by complementary binding to a miRNA of interest.Knockdown of miR-188-3p in cells by miR-188-30 sponge eliminated 2-AG-induced suppression of BACE1,where as 2-AG still suppressed BACE1 expression in cells treated with the scramble controls. These results indicate that the suppression of BACE1 by 2-AG is mediated through miR-188-3p.
Annotations
External Annotation for miR-188-3p sponge |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
