Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieColorectal Cancer
CeRNA1miR221 sponge[Artifically engineered RNA]
miRNAmiR-221[miRNA]
CeRNA2RELA[mRNA]
Tissue/Cell lineHct116 And Rko Cell Lines
SpecieHomo sapiens (human)
CitationGastroenterology 2014 Oct 147, 847-859.e11 doi:10.1053/j.gastro.2014.06.006 PMID:24931456
Reference title
A microRNA 221- and 222-mediated feedback loop maintains constitutive activation of NFkB and STAT3 in colorectal cancer cells.
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
Colorectal cancer cell lines were transfected with MIR221 or MIR222 sponges. The activity levels of NFKB and STAT3 were measured in dual luciferase reporter assays. Immunoblot and real-time PCR analyses to measure protein and mRNA levels. Cells were analyzed by proliferation, viability, and flow cytometry analyses. In agreement with the causal roles of NFKB and STAT3 in CAC development, our data vevealed dramatically decreased levels of RELA and STAT3 protein expression in tumors from mice treated with the MIR221/222 sponge. Intriguingly, treatment with the MIR221/222 sponge significantly inhibited colitis, as indicated by body weight loss, colon shortening and inflammatory factor production.
Annotations
External Annotation for miR221 sponge |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
