Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieEsophageal Squamous Cancer
CeRNA1MEG3[LncRNA]
miRNAmiR-9[miRNA]
CeRNA2FOXO1[mRNA]
Tissue/Cell lineTe1, Te13, Eca109, Yes2
SpecieHomo sapiens (human)
CitationMol Cancer Res 2017 Jul 15, 800-810 doi:10.1158/1541-7786.Mcr-16-0385 PMID:28539329
Reference title
Aberrant Methylation-mediated Silencing of lncRNA MEG3 Functions as a ceRNA in Esophageal Cancer.
Experimental verification
luciferase reporter assays;qRT-PCR;Northern blot assay;Western blot assay
Functional description
We found that transfection with miR-9 mimic significantly reduced the luciferase activities of the pGL3-MEG3 reporter vector but not empty vector or mutant vector pGL3-MEG3-M1-2 in Eca109 cell line, suggesting the binding of miR-9 to these sitesmiR-9 mimic was transfected into Eca109 cells, luciferase activity was significantly repressed in each site, whereas the repressions were completely abolished when mutation was introduced in each site. Ectopically expressed MEG3, but not the mutant, reduced the level of miR-9, suggesting the sponge role of MEG3 for miR-9.
Annotations
External Annotation for MEG3 |
|
|---|---|
 |
LncRNA-associated competing triplets and functions. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
 |
Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
 |
Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
 |
Information on all annotated and predicted human genes. |
 |
Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
 |
Genome browser for vertebrate genomes. |
 |
An annotated collection of all publicly available DNA sequences. |
 |
A wiki-based platform for community curation of human long non-coding RNAs. |
 |
An integrated knowledge database dedicated to non-coding RNAs. |
 |
An integrated database of human annotated lncRNA transcripts. |
 |
Comprehensive annotations of eukaryotic long non-coding RNAs. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
 |
The catalogue of somatic mutations in cancer. |
