Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieBreast Cancer
CeRNA1miR-20 GFP sponge(pcDNA5-CMV-d2eGFP vector)[Artifically engineered RNA]
miRNAmiR-17[miRNA]
CeRNA2E2F1[mRNA]
Tissue/Cell lineHek293T
SpecieHomo sapiens (human)
CitationNat Methods 2007 Sep 4, 721-6 doi:10.1038/nmeth1079 PMID:17694064
Reference title
MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
This difference likely results from the presence of two miR-20 binding sites and one miR-18 site in the E2F1 3' UTR, plus the added inhibition of the coexpressed miR-20 family member miR-17-5. These effects were recapitulated in a luciferase assay wherein the RLuc reporter was fused to a fragment of the E2F1 UTR spanning the two miR-20 sites
Annotations
External Annotation for miR-20 GFP sponge(pcDNA5-CMV-d2eGFP vector) |
|
|---|---|
 |
LncRNA-associated competing triplets and functions. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
 |
Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
 |
Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
 |
Information on all annotated and predicted human genes. |
 |
Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
 |
Genome browser for vertebrate genomes. |
 |
An annotated collection of all publicly available DNA sequences. |
 |
A wiki-based platform for community curation of human long non-coding RNAs. |
 |
An integrated knowledge database dedicated to non-coding RNAs. |
 |
An integrated database of human annotated lncRNA transcripts. |
 |
Comprehensive annotations of eukaryotic long non-coding RNAs. |
 |
Comprehensive experimentally supported associations between lncRNA and human cancer. |
 |
A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
 |
The catalogue of somatic mutations in cancer. |
