Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieBreast Cancer
CeRNA1miR-20 GFP sponge(pcDNA5-CMV-d2eGFP vector)[Artifically engineered RNA]
miRNAmiR-17[miRNA]
CeRNA2E2F5[mRNA]
Tissue/Cell lineHek293T
SpecieHomo sapiens (human)
CitationNat Methods 2007 Sep 4, 721-6 doi:10.1038/nmeth1079 PMID:17694064
Reference title
MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays
Functional description
we used a luciferase reporter regulated by a large fragment of the CD69 3' UTR17 or by the E2F5 UTR. As predicted by the TargetScan 4.0 and miRanda algorithms, respectively, these UTRs are each regulated by a single miR-20 site18,19. Correspondingly, each reporter was derepressed upon treatment with a miR-20 sponge in 293T cells
Annotations
External Annotation for miR-20 GFP sponge(pcDNA5-CMV-d2eGFP vector) |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
