Basic Information
Regular Relationship :
Phenotype/DiseaseSpecieNA
CeRNA1TuD-miR21-4ntin[Artifically engineered RNA]
miRNAmiR-21[miRNA]
CeRNA2PDCD4[mRNA]
Tissue/Cell linePa-1 Cells
SpecieHomo sapiens (human)
CitationNucleic Acids Res 2009 Apr 37, e43 doi:10.1093/nar/gkp040 PMID:19223327
Reference title
Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells
Experimental verification
Western blot;qRT-PCR;luciferase reporter assays;Northern blot
Functional description
we tested various RNA decoys which were designed to efficiently expose indigestible complementary RNAs to a specific miRNA molecule. These inhibitory RNAs were at the same time designed to be expressed in lentiviral vectors and to be transported into the cytoplasm after transcription by RNA polymerase III. We report the optimal conditions that we have established for the design of such RNA decoys (we term these molecules TuD RNAs; tough decoy RNAs).
Annotations
External Annotation for TuD-miR21-4ntin |
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LncRNA-associated competing triplets and functions. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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Infer genomic variations that disturb lncRNA-associated ceRNA regulation.. |
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Provide and annotate disease or phenotype-associated variants in human long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) or their regulatory elements. |
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Providing cellular-specific lncRNA-associated ceRNA networks predicted via high-throughput analysis of single-cell genomic data. |
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Information on all annotated and predicted human genes. |
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Gene nomenclature, gene families and associated resources (genomic, proteomic, phenotypic information). |
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Genome browser for vertebrate genomes. |
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An annotated collection of all publicly available DNA sequences. |
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A wiki-based platform for community curation of human long non-coding RNAs. |
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An integrated knowledge database dedicated to non-coding RNAs. |
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An integrated database of human annotated lncRNA transcripts. |
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Comprehensive annotations of eukaryotic long non-coding RNAs. |
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Comprehensive experimentally supported associations between lncRNA and human cancer. |
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A comprehensive, authoritative compendium of human genes and genetic phenotypes. |
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The catalogue of somatic mutations in cancer. |
