Breast Cancer

LINC00261[LncRNA]

miR-550a-3p[miRNA]

SDPR[mRNA]

MCF-7 cells

Homo sapiens (human)

IUBMB Life. 2021 Jan;73(1):188-201. doi: 10.1002/iub.2416. Epub 2020 Dec 3.

LINC00261/microRNA-550a-3p/SDPR axis affects the biological characteristics of breast cancer stem cells.

qRT-PCR

Long non-coding RNAs (lncRNAs) have been shown to play key roles in the pathogenesis of breast cancer (BC). The study was to explore the effect of long non-coding RNA LINC00261/microRNA (miR)-550a-3p/serum deprivation response (SDPR) axis on the biological characteristics of BC stem cells (BCSCs). BC and adjacent normal tissues of patients were collected. LINC00261, miR-550a-3p and SDPR expression in BC tissues and cell lines and CD24 and CD44 expression in BC tissues was detected. CD44(+) /CD24(-/low) BCSCs were sorted. CD44(+) /CD24(-/low) MDA-MB-231 and MCF-7 cells were screened and transfected with altered expression of LINC00261 or miR-550a-3p to explore their roles in cell viability, microsphere (MS) formation ability, migration and invasion of CD44(+) /CD24(-/low) BCSCs. The targeting relationships of LINC00261, miR-550a-3p and SDPR were detected. Reduced LINC00261, decreased SDPR and elevated miR-550a-3p exhibited in BC tissues of patients and cell lines. Elevated CD44(+/) CD24(-) were present in BC tissues. LINC00261 up-regulated SDPR expression as a sponge of miR-550a-3p. Elevated LINC00261 suppressed BC cell viability, MS formation ability, migration and invasion of CD44(+) /CD24(-/low) BCSCs. Moreover, up-regulated miR-550a-3p reversed the inhibitive effect of elevated LINC00261 on BCSCs, and reduced SDPR reversed the promotive effect of decreased miR-550a-3p on BCSCs. The study highlights that LINC00261 can adsorb miR-550a-3p to modulate SDPR, thus inhibiting the viability and MS formation of BC cells, reducing migration and invasion of CD44(+) /CD24(-/low) BCSCs, exerting a potential effect on therapy.