| Disease/Trait | breast cancer |
| LncRNA Target Gene | PVT1 |
| Tissue | cell lines (MDA-MB-231 and MCF-7) |
| Experiment | RNA-seq, qPCR, Western blot, Luciferase reporter assay etc. |
| Function Description | We show that the PVT1 promoter has a tumor-suppressor function that is independent of PVT1 lncRNA. CRISPR interference of PVT1 promoter enhances breast cancer cell competition and growth in vivo. The promoters of the PVT1 and the MYC oncogenes, located 55 kb apart on chromosome 8q24, compete for engagement with four intragenic enhancers in the PVT1 locus, thereby allowing the PVT1 promoter to regulate pause release of MYC transcription. PVT1 undergoes developmentally regulated monoallelic expression, and the PVT1 promoter inhibits MYC expression only from the same chromosome via promoter competition. Cancer genome sequencing identifies recurrent mutations encompassing the human PVT1 promoter, and genome editing verified that PVT1 promoter mutation promotes cancer cell growth. |
| Pubmed ID | 29731168 |
| Year | 2018 |
| Title | Promoter of lncRNA Gene PVT1 Is a Tumor-Suppressor DNA Boundary Element. |