Basic information of Nos2 :
| Official Symbol of Gene | Nos2 |
| Species | Mus musculus |
| Entrez Gene ID | 18126 |
| Official Full Name | nitric oxide synthase 2, inducible |
| Also known as | iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS |
| Gene Type | protein coding |
| dbXrefs | Ensembl:ENSMUSG00000020826 AllianceGenome:MGI:97361 |
| Map Location | 11 B5; 11 46.74 cM |
Sample information of multiple sclerosis:
| Detected Sample | brain |
| Sample Detail | Neonate Astrocytes |
| Detected Method | immunoblot analysis |
| Disease | stroke, multiple sclerosis, Parkinson’s disease, and HIV dementia |
| Disease subtype | N/A |
| Population | N/A |
| Sample Size | n = 5–6 mice group |
Literature information of multiple sclerosis :
| Pubmed ID | 12655043 |
| Year | 2002 |
| Title | Translational control of inducible nitric oxide synthase expression by arginine can explain the arginine paradox |
Results of multiple sclerosis :
| Expression | up-regulation |
| Risk type | Disease risk |
| Result | we demonstrate that, as expected, iNOS activity in astrocytes is governed by arginine transported into the cell from the extracellular medium. Unexpectedly, however, we found that arginine concentration not only regulates NO production by limiting availability of substrate for iNOS, it also regulates iNOS expression via translational control of iNOS mRNA |
| Mechanism/Pathway | inhibition of iNOS activity by arginine depletion in stimulated astrocyte cultures occurs via inhibition of translation of iNOS mRNA. After stimulation by cytokines, uptake of L-arginine negatively regulates the phosphorylation status of the eukaryotic initiation factor (eIF2a), which, in turn, regulates translation of iNOS mRNA. eIF2a phosphorylation correlates with phosphorylation of the mammalian homolog of yeast GCN2 eIF2a kinase. As the kinase activity of GCN2 is activated by phosphorylation, these findings suggest that GCN2 activity represents a proximal step in the iNOS translational regulation by availability of L-arginine. These results provide an explanation for the arginine paradox for iNOS and define a distinct mechanism by which a substrate can regulate the activity of its associated enzyme |
