Basic information of App :

Official Symbol of Gene	App
Species	Mus musculus
Entrez Gene ID	11820
Official Full Name	amyloid beta (A4) precursor protein
Also known as	Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
Gene Type	protein coding
dbXrefs	Ensembl:ENSMUSG00000022892 AllianceGenome:MGI:88059
Map Location	16 C3.3; 16 46.92 cM
Drug	IFNβ
Interaction Type	activor

Sample information of multiple sclerosis:

Descent	Adult male Wistar rats
Disease	AD

Literature information of multiple sclerosis :

Pubmed ID	31233762
Year	2019
Title	Intranasal interferon beta improves memory and modulates inflammatory responses in a mutant APP-overexpressing rat model of Alzheimer’s disease

Results of multiple sclerosis :

Result

Our results showed that over-expression of mutant human APP gene in the hippocampus led to learning and memory deficits concomitant with gliosis and pro-inflammatory responses.Interestingly, treatment of AD-modeled rats with IFNβ ameliorated memory impairments possibly through suppressing gliosis and shifting from pro-inflammatory toward anti-inflammatory status, suggesting that IFNβ may be a promising therapeutic agent to improve cognitive functions and modulate inflammatory responses in an AD-like neurodegenerative context.

Mechanism/pathway

Despite the fact that GFP expression level was lower than APP expression level, the lentiviral system used in this study resulted in an effective and widespread transduction of hippocampal neurons. Difference observed in expression of GFP and APP genes may be due to the particular position of these genes in FsynIGW lentiviral vector. As shown in Fig. 2A, APP gene sequence is upstream of IRES2-GFP sequence. IRES2 sequence allows bicistronic expression of APP and GFP under a neuron-specific promoter, synapsin. It has been indicated that transduced cells with a variety of bicistronic LV strongly express upstream gene of IRES sequence, whereas GFP expression (downstream gene of IRES sequence) is very low or undetectable in these cells