To assess the relevance of miR-21 in MB biology we first determined its expression level comparing to normal brain tissue in 29 MB patient’s samples as well as in a representative panel of 6 MB cell lines using quantitative reverse transcriptasepolymerase chain reaction (qRT-PCR). Interestingly, compared with normal human cerebellum, miR-21 expression was found to be up-regulated in every MB sample and MB cell line tested, with its level between 10- to 130-fold higher in MB cell nucleotide referred to as scrambled.
NSDNA: The Nervous System Disease NcRNAome Atlas
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