NSDNA: The Nervous System Disease NcRNAome Atlas

Details

Disease Name	Medulloblastoma
Nocoding RNA Name	hsa-miR-218
Expression Pattern	down-regulated
Species	Homo sapiens
Detection Methods	qRT-PCR,Luciferase Reporter Assay etc.
Target	SH3GL1
Tissue	medulloblastoma cell lines
Treatment	N/A
PubMed ID	23970061
Year	2013
Detail Description	the expression of miR-218 and SH3GL1 were assessed in four MB cell lines and normal cerebellum by qPCR. The ectopic expression of miR-218 induced by lentiviral transfection in MB cells on proliferation was evaluated by MTT assay, and cell migration and invasion were determined by transwell assays. Analysis of the target protein expression and related protein expression was determined by western blot analysis. The targeting of SH3GL1 by miR-218 was identified using a luciferase reporter assay.

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