| Detail Description |
Using FACS sorting of low-passage cell samples followed by microRNA microarray analysis, we found 43 microRNAs that were dys-regulated in common in three separate CD133+human glioblastomas compared to CD133+normal neural stem cells.we next measured miR-582-5p and miR-363 via qPCR in many GBM and three normal human tissue specimens. We found that miR-582-5p was increased in six GBMs, up to ten fold relative to the normal samples.miR-363 was increased, sometimes to extremely high levels, in nine human specimens as compared to three averaged normal brain samples. miR-582-5p and miR-363 are shown to directly target Caspase 3, Caspase 9, and Bim. |