NSDNA: The Nervous System Disease NcRNAome Atlas

Details

Disease Name	Medulloblastoma
Nocoding RNA Name	hsa-miR-34a
Expression Pattern	up-regulated
Species	Homo sapiens
Detection Methods	RT-PCR,Luciferase Reporter Assay etc.
Target	MAGE-A
Tissue	medulloblastoma cell lines
Treatment	N/A
PubMed ID	21177782
Year	2011
Detail Description	To establish the expression of miR-34a in medulloblastoma, we performed quantitative real-time PCR(q-RT-PCR) for miR-34a on a series of human medulloblastoma cell lines and primary tumors. We found 25-60-fold higher miR-34a expression in D384, D283,D341, and D556 cell lines relative to UW228, R300,and UW426 cell lines.In primary medulloblastoma samples, we confirmed the variable miR-34a expression and its correlation with p53.Cotransfection of wild-type MAGE-A 3′UTRs with pre-miR-34a, but not with the scrambled miRNA control, decreases the luciferase activity of all 4 MAGE-A constructs by more than 65%, the exception being MAGE-A2 where there is a 40% reduction.

Contact wanglh211@163.com for inquiries and feedback