| Details |
| Disease Name | Medulloblastoma |
| Nocoding RNA Name | hsa-miR-34a |
| Expression Pattern | up-regulated |
| Species | Homo sapiens |
| Detection Methods | RT-PCR,Luciferase Reporter Assay etc. |
| Target | MAGE-A |
| Tissue | brain(brain tumor tissue) |
| Treatment | N/A |
| PubMed ID | 21177782 |
| Year | 2011 |
| Detail Description | To establish the expression of miR-34a in medulloblastoma, we performed quantitative real-time PCR(q-RT-PCR) for miR-34a on a series of human medulloblastoma cell lines and primary tumors. We found 25-60-fold higher miR-34a expression in D384, D283,D341, and D556 cell lines relative to UW228, R300,and UW426 cell lines.In primary medulloblastoma samples, we confirmed the variable miR-34a expression and its correlation with p53.Cotransfection of wild-type MAGE-A 3′UTRs with pre-miR-34a, but not with the scrambled miRNA control, decreases the luciferase activity of all 4 MAGE-A constructs by more than 65%, the exception being MAGE-A2 where there is a 40% reduction. |
NSDNA: The Nervous System Disease NcRNAome Atlas
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